AntiProtech NATURE&SCIENCE

For Research Use Only (RUO). Western Blot Expert.

Claudin 5 Antibody-PA210507

Description

Claudin-5 is a member of the claudin family of integral membrane tight junction proteins. It is encoded by the CLDN5 gene located on chromosome 22q11.21. Claudin-5 is expressed predominantly in endothelial cells and is a critical component of the blood-brain barrier (BBB), where it regulates paracellular permeability and maintains barrier integrity. It also plays essential roles in the formation of tight junction strands in epithelial and endothelial cell sheets. Alterations in claudin-5 expression or localization are associated with various pathological conditions including stroke, brain tumors, cerebral ischemia, and neuroinflammatory disorders. Claudin-5 is also implicated in the regulation of cell proliferation, migration, and permeability in non-endothelial tissues.

Cata.No.: PA210507

Host: Rabbit

Reactivity: Human, Mouse, Rat

Unip.No.: O00501

M.W.: 23 kDa

Purification: Affinity Purification

Clonality: Polyclonal

Isotype: IgG

Conjugation: Unconjugated

Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

General Information

Specificity

Claudin 5 Antibody detects endogenous levels of total claudin-5 protein.

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-218 of human Claudin-5 (NP_003268.2).

Applications

WB: 1:500 - 1:2000

IHC-P: 1:50 - 1:200

IF/ICC: 1:50 - 1:200

ELISA: N/A

IP: 1:50 - 1:200

CHIP: N/A

Storage

Store at -20°C. Stable for 12 months from date of receipt.

Target Protein Information

Gene ID: 7122

Uniprot No.: O00501

Alternative Names

AWAL; BEC1; Claudin-5; CLDN5; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome.

Cellular Localization

Cell membrane; Multi-pass membrane protein. Cell junction; Tight junction. Lateral cell membrane. Localizes to tight junctions in endothelial cells and some epithelial cells.

Function

Claudin-5 is an integral membrane protein that serves as a major cell adhesion molecule at tight junctions. It plays a critical role in determining and regulating the permeability properties of endothelial barriers, particularly the blood-brain barrier (BBB). Claudin-5 forms homodimers and heterodimers with other claudin family members to create the continuous tight junction seal that restricts paracellular diffusion. It is essential for normal vascular development and angiogenesis. Downregulation or disruption of claudin-5 expression leads to increased barrier permeability, which is a hallmark of inflammation, edema, and tumor progression.

Reference Information

[1]. Morita K, Sasaki H, Furuse M, Tsukita S. Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells. J Cell Biol. 1999;147(1):185-194. doi:10.1083/jcb.147.1.185

[2]. Nitta T, Hata M, Gotoh S, et al. Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice. J Cell Biol. 2003;161(3):653-660. doi:10.1083/jcb.200302070

[3]. Greene C, Campbell M. Tight junction modulation of the blood-brain barrier: CNS delivery of small molecules. Tissue Barriers. 2016;4(1):e1138017. doi:10.1080/21688370.2015.1138017

Product Image

Western Blot Sample: HUVEC, bEnd.3, and Rat brain microvascular endothelial cell lysates, 1:1000.

IHC-P Sample: Human brain tissue, Mouse brain tissue, 1:100.

IF/ICC Sample: bEnd.3 cells, 1:100.